本研究采用多种色谱技术从一株香果树内生真菌Penicillium spinulosum大米发酵产物中获得7个次级代谢产物,经核磁、质谱等波谱学方法结合文献数据对这些化合物进行了结构鉴定,分别为spinulacid (1),ascomindone A (2),ascomindone C (3),monomethylsulochrin (4),barceloneic acid A (5),flufuran (6)和5-hydroxymethylfuraldehyde (7),其中化合物1为新苯甲酸类衍生物。药理活性评价显示,化合物1、2、5和6在20和40μmol·L^(-1)浓度下对转基因斑马鱼具有显著的促血管生成活性(动物实验获得山东省科学院生物研究所实验动物福利伦理委员会批准,批准号为SWS20240611);化合物1和4对禾谷镰刀菌表现出中等或强的抗真菌活性,其最小抑菌浓度(MIC)分别为12.5和6.25μg·mL^(-1);此外,化合物4对金黄色葡萄球菌和欧文杆菌也显示出明显的抗菌活性,其MIC值分别为6.25和12.5μg·mL^(-1)。
Intense bacterial infection,long-term inflammatory infiltration,and inadequate vascularization make diabetic wounds non-healing.Endogenous electric fields are the basis of bioelectric signal conduction and have been shown to be the primary signal guiding cell migration and promoting tissue repair.Still,the disorder microenvironment of diabetic wounds may affect the functions of endogenous electric fields.Traditional wound dressings,such as gauzes and bandages,lead to unsatisfactory repair due to their limited infection management and inability to couple with endogenous electrical fields.In this study,we develop the PMQG hydrogel,a multifunctional hydrogel dressing with effective antibacterial properties and good electroactivity,made from acrylic acid,quaternary ammonium chitosan,and MXene nanosheets.Inspired by skin,the PMQG hydrogels have flexible mechanical properties matched to the skin,strong tissue adhesion,broad-spectrum antibacterial activity,and desirable conductivity,which could transmit electrical signals,facilitating cell migration,and thus promoting the process of wound repair.The PMQG hydrogels exhibited good antibacterial properties against Escherichia coli(E.coli),Staphylococcus aureus(S.aureus),and methicillin-resistant S.aureus(MRSA),effectively controlling the infection-induced inflammation.Furthermore,incorporating MXene nanosheets into the hydrogel network enhances its reactive oxygen species scavenging ability and provides biomimetic conductivity.These anti-inflammatory properties,combined with its conductivity,help regulate the microenvironment and rebuild the endogenous electric fields,facilitating cell migration,angiogenesis,and collagen deposition,leading to a remarkable 98%wound closure by day 15 in diabetic rats,thus demonstrating superior efficacy.This novel wound dressing is expected to be an ideal therapeutic strategy for diabetic wound healing.
